Identification of immunoreactive proteins ofChlamydia trachomatis by Western blot analysis of a two-dimensional electrophoresis map with patient sera

1999 ◽  
Vol 20 (11) ◽  
pp. 2269-2279 ◽  
Author(s):  
Maria Sanchez-Campillo ◽  
Luca Bini ◽  
Maurizio Comanducci ◽  
Roberto Raggiaschi ◽  
Barbara Marzocchi ◽  
...  
Keyword(s):  
Western Blot ◽  
Blot Analysis ◽  
Western Blot Analysis ◽  
Two Dimensional ◽  
Ofchlamydia Trachomatis ◽  
Two Dimensional Electrophoresis ◽  
Dimensional Electrophoresis

Mapping ofChlamydia trachomatis proteins by Immobiline-polyacrylamide two-dimensional electrophoresis: Spot identification byN-terminal sequencing and immunoblotting

1996 ◽  
Vol 17 (1) ◽  
pp. 185-190 ◽  
Author(s):  
Luca Bini ◽  
Maria Sanchez-Campillo ◽  
Annalisa Santucci ◽  
Barbara Magi ◽  
Barbara Marzocchi ◽  
...  
Keyword(s):  
Two Dimensional ◽  
Ofchlamydia Trachomatis ◽  
Two Dimensional Electrophoresis ◽  
Dimensional Electrophoresis

scholarly journals A comparison of antigenic peptides in muscle larvae of severalTrichinellaspecies by two-dimensional western-blot analysis with monoclonal antibodies

Parasite ◽  
2001 ◽  
Vol 8 ◽  
pp. S117-S119 ◽  
Author(s):  
M.A. Dea-Ayuela ◽  
F.M. Ubeira ◽  
A. Pitarch ◽  
C. Gil ◽  
A.R. Martínez-Fernández ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Western Blot ◽  
Blot Analysis ◽  
Western Blot Analysis ◽  
Antigenic Peptides ◽  
Two Dimensional ◽  
Muscle Larvae

scholarly journals CRALBP is a Highly Prevalent Autoantigen for Human Autoimmune Uveitis

2007 ◽  
Vol 2007 ◽  
pp. 1-6 ◽  
Author(s):  
Cornelia A. Deeg ◽  
Albert J. Raith ◽  
Barbara Amann ◽  
John W. Crabb ◽  
Stephan R. Thurau ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Western Blot ◽  
Blot Analysis ◽  
Western Blot Analysis ◽  
Two Dimensional ◽  
Autoimmune Uveitis ◽  
Equine Recurrent Uveitis ◽  
And Control ◽  
Normal Controls ◽  
Subsequent Identification

Cellular retinaldehyde binding protein (CRALBP) is an autoantigen in spontaneous equine recurrent uveitis. In order to test whether CRALBP contributes to human autoimmune uveitis, the specificity of antibodies from human uveitis patient's sera was first evaluated in two-dimensional (2D) Western blot analysis. Subsequent identification of the immunoreactive proteins by mass spectrometry resulted in the identification of CRALBP as a putative autoantigen. Additionally, sera from human uveitis and control patients were by Western blot using purified human recombinant CRALBP. Anti-CRALBP autoantibodies occur more frequently (P<.01) in human uveitis patients than in normal controls. Thirty out of 56 tested uveitis patient's sera contained autoantibodies reactive against CRALBP, compared to only four out of 23 normal control subjects. The presence of CRALBP autoantibodies in 54% of tested uveitis patients supports CRALBP as a possible autoantigen in human autoimmune uveitis.

scholarly journals Two-Dimensional Gel-Based Protein Standardization Verified by Western Blot Analysis

2015 ◽  
pp. 473-479 ◽  
Author(s):  
Hisao Haniu ◽  
Daisuke Watanabe ◽  
Yusuke Kawashima ◽  
Hiroyuki Matsumoto
Keyword(s):  
Western Blot ◽  
Blot Analysis ◽  
Western Blot Analysis ◽  
Two Dimensional
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